Safron (Crocus sativus) is widely used as an important herbal medicine crop worldwide. Iran is the largest producer and exporter of safron in the world. Turnip mosaic virus (TuMV) is a species of the genus Potyvirus, which infects a wide range of plants species. During the autumn of 2019, safron plants with symptoms including colour breaking of the fowers, chlorosis and mild mosaic of leaves were collected from the major safron cultivation areas of Iran. After the isolation of total RNA from diseased samples, RT-PCR was done using TuMV-specifc primers. Amplicons of two selected isolates were purifed, cloned, and sequenced. BLASTn of obtained sequences indicated high similarity with TuMV sequences. To the best of our knowledge, this is the frst fnding of TuMV infection in the safron plant of Iran.

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