Dust particles (DPs) are one of the most important public health concerns in the urban environment. The presence of heavy metals (HMs) on the surface of DPs might increase the health risk of exposure to the DPs. Accordingly, The purpose of this study was to examine the content of HMs in the outdoor and indoor DPs in Neyshabur city and assess the cytotoxic effects of DPs exposure on lung, gastric, and skin cell lines. To this end, the city was divided into three areas, high-traffic, medium-traffic, and low-traffic (rural). The average concentration of the HMs in the indoor DPs were as follows, 655.5 μg g-1 for Zn, 114.6 μg g-1 for Cu, 77.7 μg g-1 for Cr, 108.6 μg g-1 for Ni, 52 μg g-1 for Pb, 12 μg g-1 for Co, and 3.3 μg g-1 for Cd, while the average concentration of Zn, Cu, Cr, Ni, Pb, Co, Cd in the outdoor DPs were 293.7 μg g-1, 200.6 μg g-1, 100.7 μg g-1, 68.4 μg g-1, 44.7 μg g-1, 18.6 μg g-1, 0.25 μg g-1, respectively. A higher concentration of HMs, as well as cytotoxicity, were revealed in the indoor samples compared to outdoor ones. The degree of cytotoxicity of DPs collected from high-traffic areas was higher than that of low and medium-traffic ones. In addition, treatment of AGS and L929 cells with indoor dust samples induced the expression level of inflammatory agents such as TNFα, IL6, and, CYP1A1 genes more than in outdoor dust samples (P < 0.05). Briefly, a higher level of HMs concentration and cytotoxicity effect on the given cell lines was observed in the samples taken from indoor environments and high-traffic areas.

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