Microencapsulation is a common method to improve the viability of probiotic bacteria against environmental stresses. In this research, by using double emulsion with Persian gum, emulsion stability, physicochemical properties, microcoating efficiency and microcoating viability were investigated during storage and in a simulated gastrointestinal conditions of the digestive system. Encapsulation of Lactobacillus acidophilus in double emulsion (W/O/W) with Persian gum improved the survival under storage conditions at 4 °C for 28 days, and encapsulation treatment only 0.58 log decreased and the treatment without capsules reached zero after 7 days. The microcoating efficiency was 88% and the emulsion stability was between 85% and 95.43%. The optical microscope image showed a distinct double emulsion. In the simulating conditions of the stomach, the number of bacteria for encapsulation treatment decreased by 1.97 log and the treatment without capsules 3.9 log, and in the simulating conditions of the intestine, for encapsulation treatment it decreased by 0.45 log and for the treatment without capsules 1.3 log decreased. The particle size, zeta potential and dispersion index was 525 nm, -44.68 mv and 0.33 respectively. The results showed that the use of Persian gum double emulsion improves the survival of Lactobacillus acidophilus against storage conditions and the simulated environment of the digestive system.

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