Abstract: In the present study, the effects of ultrasound as emerging technology were investigated on the alpha-amylase activity of barley seeds after sowing along with the normal watering of seeds. The seeds of barley after steeping were exposed to ultrasonic irradiation in vibration amplitude controller setting 20, 60 and 100% of nominal power for times (5, 10 and 15 min) at 20 KHz and 30°C. For determining the effects of these parameters on enzyme the Fuwa method assay based on the decreased staining value of blue starch-iodine complexes employed for measurement an activity. The results of these assays were analysed by Qualitek4 software using the Taguchi statistical method to evaluate the factor’s effects on enzyme activity. It has been recorded; an increase in enzyme activity at the lower power densities followed a decrease in activity at the higher power densities employed

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