The purpose of this study was to define the structures of the digits and hoof in Holstein dairy cattle by using computed tomography scan (CT scan). Transverse, sagital and dorsoplantar CT images of two isolated cattle cadaver digits were obtained using a Siemens ARTX2 Somatom. The CT images were compared to corresponding frozen crosssections. Relevant anatomical structures were identified and labelled at each level. The CT images provided anatomical detail of the digits and hoof in Holstein dairy cattle. Transversal images provided excellent depiction of anatomical structures when compared to corresponding frozen crosssections. The information presented in this paper would serve as an initial reference to the evaluation of CT images of the digits and hoof in Holstein dairy cattle.

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