Iranian Journal of Science and Technology-Transaction A: Science, ( ISI ), Volume (31), No (3), Year (2008-1) , Pages (263-270)

Title : ( Debittering of tryptic digests from beta casein and enzyme modified cheese by X-PDP )

Authors: Mohammad B Habibi Najafi , ب لی ,

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The proline-rich -casein was digested in vitro with trypsin, and the oligopeptides produced were then isolated by RP-HPLC and subsequently identified by amino acid analysis and ion mass spectrometry. The peptide fractions from the complete digestion were then treated with purified x-prolyl dipeptidyl peptidase (X-PDP) extracted from Lactobacillus casei ssp. casei LLG. Two bitter peptides (f53-97 and f203-209) containing X-Pro-Y-Pro in their amino acid residues were completely hydrolyzed by X-PDP while several peptides with a high degree of hydrophobicity were also decreased in peak area. Debittering effect of X-PDP from Lactobacillus casei ssp. casei LLG on enzyme modified cheese (EMC) was also investigated both by subjective and objective methods. The bitterness of Cheddar cheese slurries supplemented with Neutrase® 0.5 L was completely eliminated after treatment with crude enzyme extract from Lactobacillus casei ssp. casei LLG. Two hydrophobic peptides in EMC with Ala-Pro-Phe-Pro-Glu-Val, and Phe-Leu-Leu residues were hydrolyzed by crude enzyme extract. The RP-HPLC and subsequently ion mass spectrometry analysis have shown that the debittering effect on EMC was due partially to the presence of X-PDP.

Keywords

, (Key words: Debittering, -casein, enzyme modified cheese, X-Prolyl Dipeptidyl Peptidase,
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@article{paperid:1006573,
author = {Habibi Najafi, Mohammad B and ب لی},
title = {Debittering of tryptic digests from beta casein and enzyme modified cheese by X-PDP},
journal = {Iranian Journal of Science and Technology-Transaction A: Science},
year = {2008},
volume = {31},
number = {3},
month = {January},
issn = {1028-6276},
pages = {263--270},
numpages = {7},
keywords = {(Key words: Debittering; -casein; enzyme modified cheese; X-Prolyl Dipeptidyl Peptidase; lactobacilli).},
}

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%0 Journal Article
%T Debittering of tryptic digests from beta casein and enzyme modified cheese by X-PDP
%A Habibi Najafi, Mohammad B
%A ب لی
%J Iranian Journal of Science and Technology-Transaction A: Science
%@ 1028-6276
%D 2008

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