Title : ( Genetic Diversity of Sinorhizobium meliloti Isolated from RootNodules of Alfalfa (Medicago sativa) Growing in Hamadan Soils )
Authors: Amir Lakzian , esmaeil karimi , خاوازی کاظم , Gholam Hosain Haghnia ,
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Abstract
Genetic diversity of 196 isolates of Sinorhizobium from nodules of Medicago sativa (cv. Hamadani) grown in different soil samples was investigated. Plasmid profile and the PCR/RFLP of 16S-23S rRNA were employed for this study. The results of the plasmid profile distinguished 27 groups among 196 isolates. PCR/RFLP of 16S-23S rRNA from 150 isolates of Sinorhizobium showed that they could be divided into three distinct groups. The two major groups were identified as S. meliloti and S. medicae and a third minor group appeared different from the other two. Shannon’s index was different in each land type in Hamadan province. Results also indicated that there was a positive correlation between some soil characteristics and Shannon’s index.
Keywords
Diversity; Sinorhizobium; Plasmid profiles; PCR/RFLP; 16SrRNA
@article{paperid:1006741,
author = {Lakzian, Amir and Karimi, Esmaeil and خاوازی کاظم and Haghnia, Gholam Hosain},
title = {Genetic Diversity of Sinorhizobium meliloti Isolated from RootNodules of Alfalfa (Medicago sativa) Growing in Hamadan Soils},
journal = {International Journal of Agriculture and Biology},
year = {2008},
volume = {10},
number = {6},
month = {February},
issn = {1560-8530},
pages = {669--672},
numpages = {3},
keywords = {Diversity; Sinorhizobium; Plasmid profiles; PCR/RFLP; 16SrRNA},
}
%0 Journal Article
%T Genetic Diversity of Sinorhizobium meliloti Isolated from RootNodules of Alfalfa (Medicago sativa) Growing in Hamadan Soils
%A Lakzian, Amir
%A Karimi, Esmaeil
%A خاوازی کاظم
%A Haghnia, Gholam Hosain
%J International Journal of Agriculture and Biology
%@ 1560-8530
%D 2008
