Phytomedicine, ( ISI ), No (16), Year (2009-3) , Pages (181-187)

Title : ( Mogoltacin enhances vincristine cytotoxicity in human transitional cellcarcinoma (TCC) cell line )

Authors: Fatemeh Behnam Rassouli , Maryam Moghaddam Matin , مهرداد ایرانشاهی , Ahmad Reza Bahrami , وجیهه نشاطی , سمانه ملازاده , زینب نشاطی ,

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Bladder cancer is the second common cancer of the genitourinary system throughout the world and intravesical chemotherapy is usually used to reduce tumour recurrence and progression. Human transitional cell carcinoma (TCC) is an epithelial-like adherent cell line originally established from primary bladder carcinoma. Here we report the effect of mogoltacin, a sesquiterpene coumarin from Ferula badrakema on TCC cells. Mogoltacin was isolated from the fruits of F. badrakema, using silica gel column chromatography and preparative thin layer chromatography. Mogoltacin did not have any significant cytotoxicity effect on neoplastic TCC cells at 16, 32, 64, 128, 200 and 600 mgml1 concentrations. In order to analyse its combination effect, TCC cells were cultured in the presence of various combining concentrations of mogoltacin and vincristine. Cells were then observed for morphological changes (by light microscopy) and cytotoxicity using MTT assay. The effect of mogoltacin on vincristine toxicity was studied after 24, 48 and 72 h of drug administration. The results of MTT assay showed that mogoltacin can significantly enhance the cytotoxicity of vincristine and confirmed the morphological observations. Results revealed that combination of 40 mgml1 vincristine with 16 mgml1 mogoltacin increased the cytotoxicity of vincristine after 48 h by 32.8%.

Keywords

Ferula badrakema; Mogoltacin; Vincristine; Cytotoxicity; TCC cells; Sesquiterpene coumarin; Synergy
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@article{paperid:1008259,
author = {Behnam Rassouli, Fatemeh and Moghaddam Matin, Maryam and مهرداد ایرانشاهی and Bahrami, Ahmad Reza and وجیهه نشاطی and سمانه ملازاده and زینب نشاطی},
title = {Mogoltacin enhances vincristine cytotoxicity in human transitional cellcarcinoma (TCC) cell line},
journal = {Phytomedicine},
year = {2009},
number = {16},
month = {March},
issn = {0944-7113},
pages = {181--187},
numpages = {6},
keywords = {Ferula badrakema; Mogoltacin; Vincristine; Cytotoxicity; TCC cells; Sesquiterpene coumarin; Synergy effect},
}

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%0 Journal Article
%T Mogoltacin enhances vincristine cytotoxicity in human transitional cellcarcinoma (TCC) cell line
%A Behnam Rassouli, Fatemeh
%A Moghaddam Matin, Maryam
%A مهرداد ایرانشاهی
%A Bahrami, Ahmad Reza
%A وجیهه نشاطی
%A سمانه ملازاده
%A زینب نشاطی
%J Phytomedicine
%@ 0944-7113
%D 2009

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