Cell proliferation, ( ISI ), Volume (41), Year (2008-2) , Pages (28-35)

Title : ( Knocking-down the expression of nucleostemin significantly decreases rate of proliferation of rat bone marrow stromal stem cells in an apparently p53-independent manner )

Authors: S. M. Jafarnejad , S. J. Mowla , Maryam Moghaddam Matin ,

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Objectives : Nucleostemin (NS) is a recently identified GTP-binding protein, predominantly expressed in embryonic and adult stem cells but not in terminally differentiated cells. NS is expressed in bone marrow-derived mesenchymal stem cells, and its expression ceases upon induction of neural differentiation. The major aim of this study was to determine whether down-regulation of NS expression acts as a promoter, or otherwise as a by-product of differentiation and senescence processes. Materials and methods : We used RNA interference protocols to specifically knock down NS in rat bone marrow-derived stromal stem cells. Changes in rate of proliferation and cell cycle profile after knocking-down of NS were measured. In addition, changes in expression of associated genes were studied by semiquantitative RT-PCR, Western blotting and immunocytochemistery. Results : Knocked-down expression of NS caused a significant decrease in the rate of cell proliferation with concomitant shutting off of expression of cyclin D1 and survivin, two other well-known regulators of cell proliferation. Interestingly, we noticed no obvious changes in expression level of p21, the main effector of p53 for its cell cycle repressing function. Conclusion : Our findings revealed a master role for NS in promoting proliferation of rat bone marrow-derived stromal stem cells. Moreover, we suggest that despite previous proposals, the cell cycle arrest/inhibitory role of NS is unlikely to be related to its proposed property of interaction with p53.

Keywords

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@article{paperid:1009133,
author = {S. M. Jafarnejad and S. J. Mowla and Moghaddam Matin, Maryam},
title = {Knocking-down the expression of nucleostemin significantly decreases rate of proliferation of rat bone marrow stromal stem cells in an apparently p53-independent manner},
journal = {Cell proliferation},
year = {2008},
volume = {41},
month = {February},
issn = {0960-7722},
pages = {28--35},
numpages = {7},
keywords = {nucleostemin},
}

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%0 Journal Article
%T Knocking-down the expression of nucleostemin significantly decreases rate of proliferation of rat bone marrow stromal stem cells in an apparently p53-independent manner
%A S. M. Jafarnejad
%A S. J. Mowla
%A Moghaddam Matin, Maryam
%J Cell proliferation
%@ 0960-7722
%D 2008

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