A study was done to identify of tick vectors of Babesia ovis by using PCR. During spring and summer of 2006, a number of ticks were collected from sheep suspected to be infected with Babesia spp. First, the ticks were identified by diagnostic keys, then, the DNA of tick samples were extracted by DNA extraction Kit(Cinnagen). The PCR technique were done by two designed primers for detection of Babesia ovis. The results showed that just one of primers had acceptable reaction on control positive samples . Results of PCR by using with this primer were shown that 2 of 20 DNA samples of Rhipicephalus sanguienus , 7 of 13 DNA samples of Hyalomma marginatum and 4 of 7 DNA samples of Hyalomma anatolicum had 550 bp specific band on agarose gel electrophoresis. Based on the results , it seems that Hyalomma marginatum and Hyalomma anatolicum have more important in the transmission of Babesia ovis in sheep of this area.

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