5th Meeting International Weed Science Congress , 2008-06-23

Title : ( Molecular Study of Resistance to Aryloxyphenoxypropionate )

Authors: M0hammad Hassan Rashed Mohassel , رضا قربانی , Mohammad Taghi Alebrahim , فریبا مطقانی , محمد علی باغستانی , مهدی نصیری محلاتی ,

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. Molecular Study of Resistance to Aryloxyphenoxypropionate Herbicides in Winter Wild Oat (Avena ludoviciana Durieu.) Populations from Iran. Javid Gherekhloo1, Mehdi Rastgoo2, Mohammad Rashed Mohassel1, Mehdi Nassiri Mahallati1, Eskandar Zand3, Rafael De Prado4; 1Ferdowsi University of Mashhad, Mashhad, Khorasan Razavi, Iran; 2Zanjan University, Zanjan, Iran; 3Plant Protection Research Institute, Tehran, Iran; 4Universidad de Cordoba, Cordoba, Spain To molecular study of resistance to Aryloxyphenoxypropionate herbicides in winter wild oat (Avena ludoviciana Durieu.) populations, an experiment was conducted in base of dCAPS method at Biotechnology Laboratory of Ferdowsi University of Mashhad in 2006. Thirty-eight suspected populations, sampled from Ahvaz (VR), Andimeshk (NR), Shush (SOR), Shushtar (STR), Ramhormoz (ZR), Susangerd (Dashte Azadegan) (AR), and Dezful (DR) cities of Khuzestan province-Iran, were sprayed with recommended dose of clodinafop propargyl, diclofop methyl, and fenoxaprop-P ethyl herbicides, separately. Survived populations were exposed to a dose-response assay in greenhouse with the mentioned herbicides. Then the populations with high resistance factor subjected to the molecular study. dCAPS method was carried out in three steps including PCR, enzyme digestion, and gel electrophoresis. Results showed that mutation at position 1781 of ACCase enzyme resulted in a high level of resistance in 10 wild oat populations to aryloxypropionate herbicides. Substitution of isoleucine by leucine at position 1781 of acetyl CoA carboxylase is a key point mutation conferring resistance to most ACCase inhibiting herbicides in grass weeds. Other mechanisms maybe are responsible for resistance in the rest of resistant populations in this study.


, Resistance, Aryloxyphenoxypropionate, Wild Oat
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author = {Rashed Mohassel, M0hammad Hassan and رضا قربانی and Alebrahim, Mohammad Taghi and فریبا مطقانی and محمد علی باغستانی and مهدی نصیری محلاتی},
title = {Molecular Study of Resistance to Aryloxyphenoxypropionate},
booktitle = {5th Meeting International Weed Science Congress},
year = {2008},
location = {ونکوور},
keywords = {Resistance; Aryloxyphenoxypropionate; Wild Oat},


%0 Conference Proceedings
%T Molecular Study of Resistance to Aryloxyphenoxypropionate
%A Rashed Mohassel, M0hammad Hassan
%A رضا قربانی
%A Alebrahim, Mohammad Taghi
%A فریبا مطقانی
%A محمد علی باغستانی
%A مهدی نصیری محلاتی
%J 5th Meeting International Weed Science Congress
%D 2008