The major and accessory sub mandibular glands were obtained from different species of rodents belong to families Muridae (Meriones lybicus, Mus musculs, Cricetulus migraturus, Nesokia indica, Laboratory hamster and Apodemus sp.)? Dipodidae (Alactage elater and Jucullus blanfordi) and Sciuridae (Funambulus pennanti). The skulls of these species were separated immediately after death and fixed in buin with decalcification. Five um sections were stained with hematoxilen-eosin and tetrachrom. Periodic acid shiff and alcian blue (pH 1) were performed for sulfated natural mucins. Microscopic histological features, including existence of mucus and serous acini, presence of different kinds of tubules and different types of ducts as well as the histochemical characteristic features including histochemistry of different tubules and ducts in 2 different pH levels in the major and accessory sub mandibular glands in different rodents showed that the studied species proved to be different and the histological and histochemical study of sub mandibular salivary glands proved practicable with good discriminatory potential in evaluating the inter-species differences.

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