The Sugarcane grasshopper (Chrotogonus trachypterus) is one of the most important and harmful pest of field crops in Sistan region of Iran. Concerns over the environmental and human health impacts of chemical control of grasshoppers have led to considerable interest in developing of alternative control methods e.g. using entomopathogenic fungi. Effects of two native isolates of the fungus, Beauveria bassiana, a native isolate of Metarhizium anisopliae and Green Muscle, a commercialized formulation of Metarhizium anisopliae var. acridum , were investigated on the Chrotogonus trachypterus. After doing pathogenicity and bracketting tests, serial dilution for three isolates were prepared as followed : 102, 3/2102, 103,3/2103 ,104 spore/insect and 10,3/210,102,3/2102,103 spore/insect for native isolates of B.bassiana and M.anisopliae, respectively; finally, 104, 3/2104, 105, 3/2105 and 106 spore/insect were logarithmic doses for formulated product of M.anisopliae. Comparison between LD50 and LD95 off all isolates were demonstrated that native isolate of Metarhizium anisopliae had the lowest LD50 ; Other isolates had high pathogenicity rather than commercialized product .These results indicate that more studies in order to isolation of native strains of entomopathogenic fungi and determination of their effectivness on insect pests are essential

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