International Journal of Biological Macromolecules, ( ISI ), Volume (46), No (3), Year (2010-3) , Pages (289-297)

Title : ( A Novel Thermostable, Acidophilic -Amylase from a New Thermophilic -Bacillus sp. Ferdowsicous- Isolated from Ferdows Hot Mineral Spring in Iran: Purification and Biochemical Characterization )

Authors: Ahmad Asoodeh , J. Chamani , Milad Lagzian ,

Citation: BibTeX | EndNote

This paper describes the purification and characterization of a novel acidophile -amylase from newly isolated Bacillus sp. Ferdowsicous. The enzyme displayed a molecular weight of 53 kDa and it was stable over a range of pH from 3.5 to 7 with an optimum around 4.5. The optimum temperature for activity was found to be around 70 ◦C and the enzyme remained active to more than 75% up to 75 ◦C for 45 min. The enzyme activity was decreased by Zn2+andEDTAbut inhibited byHg2+, whereas the activity was increased by approximately 15% by Ba2+ and Fe2+. Na+, Mg2+, K+, Ca2+, PMSF, Triton X-100 and mercaptoethanol had any considerable effect on its activity. The enzyme activity on the amylose as substrate was 1.98 times greater than amylopectin. Partial N-terminal sequencing demonstrated no significant similarity with other known alpha-amylases, indicating that the presented enzyme was new. Considering its promising properties, this enzyme can find potential applications in the food industry as well as in laundry detergents.

Keywords

, Thermostable alpha, Amylase Purification Acidophile Bacillus sp.
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@article{paperid:1014399,
author = {Asoodeh, Ahmad and J. Chamani and Lagzian, Milad},
title = {A Novel Thermostable, Acidophilic -Amylase from a New Thermophilic -Bacillus sp. Ferdowsicous- Isolated from Ferdows Hot Mineral Spring in Iran: Purification and Biochemical Characterization},
journal = {International Journal of Biological Macromolecules},
year = {2010},
volume = {46},
number = {3},
month = {March},
issn = {0141-8130},
pages = {289--297},
numpages = {8},
keywords = {Thermostable alpha-Amylase Purification Acidophile Bacillus sp. Ferdowsicous},
}

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%0 Journal Article
%T A Novel Thermostable, Acidophilic -Amylase from a New Thermophilic -Bacillus sp. Ferdowsicous- Isolated from Ferdows Hot Mineral Spring in Iran: Purification and Biochemical Characterization
%A Asoodeh, Ahmad
%A J. Chamani
%A Lagzian, Milad
%J International Journal of Biological Macromolecules
%@ 0141-8130
%D 2010

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