Thermal denaturation of spinach plastocyanin was investigated by molecular dynamics simulation in order to study of unfolding mechanism of this protein. Molecular dynamics simulation was carried out in high temperature within 18 ns by using Gromacs software. The radius of gyration (Rg) and Root Mean Deviation (RMSD) have been used to follow the unfolding process. Moreover, distributions of Rg were used to identify states during the simulation. By using Gaussian curve fitted to distribution of Rg curves, three state and two transition ensemble was detected during the simulation.

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