Title : ( An approach based on diffusion to study ligand–macromolecule interaction )
Authors: Mohammad Reza Housaindokht , Mahmood Bahrololoom , - - , Ali A. Mossavi-Movahedi ,
Full TextAbstract
A new approach has been developed to study binding of a ligand to a macromolecule based on the diffusion process. In terms of the Fick’s first law, the concentration of free ligand in the presence of a protein can be determined by the measurement of those ligands which are diffused out. This method is applied to the study of binding of methyl-orange to lysozyme in phosphate buffer of pH 6.2, at 30C. The binding isotherm was determined initially, followed by application of the Hill equation to the data obtained, then binding constant and binding capacity were estimated.
Keywords
, binding isotherm, hen egg-white lysozyme, ligand–protein interaction, methyl orange
@article{paperid:1015080,
author = {Housaindokht, Mohammad Reza and Bahrololoom, Mahmood and -, - and Ali A. Mossavi-Movahedi},
title = {An approach based on diffusion to study ligand–macromolecule interaction},
journal = {Acta Biochimica Polonica},
year = {2002},
volume = {49},
number = {3},
month = {June},
issn = {0001-527X},
pages = {703--707},
numpages = {4},
keywords = {binding isotherm; hen egg-white lysozyme; ligand–protein interaction; methyl orange},
}
%0 Journal Article
%T An approach based on diffusion to study ligand–macromolecule interaction
%A Housaindokht, Mohammad Reza
%A Bahrololoom, Mahmood
%A -, -
%A Ali A. Mossavi-Movahedi
%J Acta Biochimica Polonica
%@ 0001-527X
%D 2002
