Abstract This study was performed to evaluate the surgical technique required and the clinical usefulness of tissue adhesive (2-Octyl cyanoacrylate) combined with amniotic membrane (AM) patching in the treatment of experimental corneal burn in dogs. Alkali wounds were inflicted on the central corneas of dogs by applying a round filter paper, 6.0 mm in diameter, soaked in 1 M NaOH for 60 s. Only one eye in each dog was used. A total of 15 dogs were divided into three groups of five animals each: (1) uncovered—control, (2) covered by AM with the amnion cell side down and secured with 10–0 nylon sutures to the cornea around the wound area—AM+suture, and (3) covered by sutureless AM patching secured with 2-Octyl cyanoacrylate (Dermabond)—AM+glue. The operating time was compared between both treatment groups. Clinical outcome was monitored by evaluation of epithelial defects, corneal opacity, duration of blepharospasm, time of AM persistence, corneal vascularisation, and duration of ocular discharge. The mean surgery time in AM+suture group was significantly longer than AM+glue group. AM persistence in AM+glue group was significantly greater than AM+ suture group. The duration of ocular discharge and corneal vascularisation in AM+glue group was significantly lower in comparison with control group. Epithelial healing was faster in the AM+glue group than in controls. In conclusion, sutureless AM patching with 2-Octyl cyanoacrylate (Dermabond) as a dressing on a corneal alkali burn, used for the first time in this research, may induce rapid epithelial healing with less vascularisation and be a much faster and useful technique in dogs

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