Glioma is the most common tumor of the central nervous system and glioblastomas are the most common and most malignant primary brain tumors. Despite combined treatment with surgery, radiotherapy and chemotherapy, the prognosis of glioblastoma remains very poor, with a median survival below 15 months, underlying the need to develop new therapeutic approaches, such as immunotherapy. It is well recognized that cell line models of human disorders, especially cancers, are an important resource for finding new therapeutic as well as biological mechanisms underlying cancer development. U87 MG cells are an in vitro model of human glioblastoma cells to investigate the drug cytotoxicity as well as finding new therapeutics towards cancer cells. In this study, we chose the human glioblastoma cell line U87 MG as an in vitro model to detect the presence of innate immunity receptors in this cells and we therefore investigated whether there was a difference between the expression of these genes in U87 MG cells and peripheral blood mononuclear cells (PBMC) of healthy individuals. In this study, the expression of several human toll like receptors(TLRs) such as TLR2, TLR4 and CD14 was quantitatively measured. Our results show that the level of CD14 expression in U87MG cells was significantly lower than the expression level of this gene in PBMC..

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