An experiment was carried out to determine the influence of feeding different levels of Locally Produced Canola Seed (LPCS) on performance and fatty acid compositions of egg yolk lipid. In a completely randomized design, four treatments including control and three levels of whole canola seed (5, 10 and 15%) were fed to 108 laying hens (Hy-line w-36 at the age of 30 weeks) in four groups with three replications for 12 weeks. Fatty acid compositions of egg yolk were measured at the end of 41 weeks. Usage of LPCS in all levels decreased total amount of saturated fatty acid and the ratio of n-6 to n-3 of the yolk in comparison with control diet. The diets had significant effects on egg shell weight, haugh unit and yolk color index. With 15% LPCS in diet, significantly decreased daily haugh unit in comparison with control diet. Supplementation of LPCS >10% in diet resulted to decreased egg shell weight and yolk color comparing to control diet. There were significant differences in daily feed intake, egg production and egg mass. There was no difference in daily produced egg mass between levels of 5 and 10% LPCS in comparison with control diet (51.94 and 50.57 vs. 51.67). The using of 10% LPCS in diet had the best result between treatments due to better performance and ratio of n-6 to n-3 polyunsaturated fatty acid in egg yolk in comparison with control diet (8.28 vs. 16.94

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