DNA and Cell Biology, ( ISI ), Volume (30), No (1), Year (2011-1) , Pages (25-31)

Title : ( Correlation Between PXR and ABCG2 Patterns of mRNA Expression in MCF7 Breast Carcinoma Cell Derivatives upon Induction by Proinflammatory Cytokines )

Authors: Obeid M. Malekshah , Ahmad Reza Bahrami , Jalil Tavakol Afshari , Fatemeh Mosaffa , Javad Behravan ,

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In this study, we aimed at evaluating the relationship between PXR and ABCG2 gene expression patterns induced by proinflammatory cytokines in MCF7 and MCF7/MX breast carcinoma cell lines. The effects of proinflammatory cytokines on ABCG2 and PXR mRNA expression were studied using real-time polymerase chain reaction method. Significant inductions in the ABCG2 and PXR mRNA levels were observed in MCF7 cells, on treatment with interleukin 1b and tumor necrosis factor-a, whereas MCF7/MX cells did not seem to significantly respond to the treatment. The results also show that in comparison to MCF7 cell line the basal mRNA expression level of PXR was higher in MCF7/MX cell line. In conclusion, interleukin-1b and tumor necrosis factor-a induced ABCG2 and PXR mRNAs in MCF7 breast cancer cell line; no significant changes on expression of the same genes in MCF7/MX cells were observed. This differential effect of the cytokines on two different cell lines seems to be influenced by basal levels of the mRNAs, which are very high in MCF7/MX cells. Similar patterns of induction in PXR and ABCG2 genes suggest a probable relationship between these two factors.

Keywords

, breast carcinoma, MCF7/MX cell
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@article{paperid:1019544,
author = {Obeid M. Malekshah and Bahrami, Ahmad Reza and Jalil Tavakol Afshari and Fatemeh Mosaffa and Javad Behravan},
title = {Correlation Between PXR and ABCG2 Patterns of mRNA Expression in MCF7 Breast Carcinoma Cell Derivatives upon Induction by Proinflammatory Cytokines},
journal = {DNA and Cell Biology},
year = {2011},
volume = {30},
number = {1},
month = {January},
issn = {1044-5498},
pages = {25--31},
numpages = {6},
keywords = {breast carcinoma;MCF7/MX cell line},
}

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%0 Journal Article
%T Correlation Between PXR and ABCG2 Patterns of mRNA Expression in MCF7 Breast Carcinoma Cell Derivatives upon Induction by Proinflammatory Cytokines
%A Obeid M. Malekshah
%A Bahrami, Ahmad Reza
%A Jalil Tavakol Afshari
%A Fatemeh Mosaffa
%A Javad Behravan
%J DNA and Cell Biology
%@ 1044-5498
%D 2011

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