Introduction: Vitreous humor is a transparent gel which fills the distance between the lens and retina in the eye of human and other vertebrates. It is a gelatin mass without color that is produced by certain cells in the retina. The density of vitreous humor is two to four times more than pure water. By weight, vitreous is ~ 99% water and 0.9% salts. The remaining 0.1% is divided between protein and polysaccharide components. NTERA2, a human embryonal carcinoma (EC) stem cell line, shares many characteristics with human embryonic stem cells (hESCs). Today, the role of hyaluronic acid on proliferation and cell migration has been proven in wound healing process. Methods: In this experiment, two T25 flask containing NT2 cells were selected and one of them was treated with retinoic acid (RA) 10-5M with concentration 1:1000. After 4 days of incubation, the cells were subcultured then 375μL of VH was added (7.5% V/V) in one of the two flasks. After four days the cells were cultured in 6-well plates. NT2 cells (as control) and cells treated with RA (without treatment with vitreous) were cultured at the same time in 6-well plates. Cells were counted after three, six and nine days using Neobar slides. Results: Results showed that number of the cells treated with retinoic acid was less than NT2 cells, while this number was partly restored upon treatment of the cells with retinoic acid and then vitreous. Conclusion: Because vitreus humor contains hyaluronic acid and it has been shown that this compound has a significant role on cell proliferation, it could be concluded that constituents of VH such as hyaluronic acid, are effective on NT2 cells proliferation. Key words:

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