4th Yazd International Congress and Student Award in Reproductive Medicine , 2011-04-15

Title : ( In vivo differentiation of Mesenchymal Stem Cells (MSCs) in rat torsion testis )

Authors: Mohammad Ali Sabbaghi Mehrjardi , Ahmad Reza Bahrami , Behzad Feizzade , Seyed Mahdi Kalantar , Maryam Moghaddam Matin , M Kalantari , Abbas Aflatoonian ,

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Introduction: Regenerative medicine has enjoyed the potential of Mesenchymal adult Stem Cells (MSCs) in therapeutical levels. Reproductive impotency as a major medical problem is being approached by stem cell therapy in recent years. Azoospermia caused by torsion in testis is a common source of impotency, which has not been touched by this approach yet. Materials and Methods: MSCs were extracted from rat bone marrow, cultured and transplanted into azoospermed testis to investigate the regenerative capacity of the cells in torsioned testis. We used germ cell specific markers (Oct4, Dazl, Vasa and c-Kit) to assess the differentiation of MSCs after transplantation into the azoospermed testis. Results: The extracted MSCs were shown to grow well in the cultured flasks. Following the successful labeling with DiI, the testis injected cells were proven to last for long time post transplantation. While expression of Oct4 and Dazl were detected 45 days after the cell treatment, Vasa and c-Kit proteins were not detectable after this period of time. 95 days after MSC transplantation, Oct4, Dazl and Vasa expression were detectable, but c-Kit remained undetectable yet. Meanwhile in predicted positions of the implanted cells it did not seem to have c-Kit expression 180 days after transplantation, and consequently the biopsies did not show any sperm formation. Conclusion: In this study, for the first time, regenerative capacity of stem cells in torsion induced azoospermia in rats was evaluated. We used MSCs transplantation to revive the spermatogenesis in torsion testis. This capacity was monitored using different molecular markers.

Keywords

, Mesenchymal Stem Cells (MSCs), In vivo differentiation, Torsion
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@inproceedings{paperid:1022278,
author = {Sabbaghi Mehrjardi, Mohammad Ali and Bahrami, Ahmad Reza and Behzad Feizzade and Seyed Mahdi Kalantar and Moghaddam Matin, Maryam and M Kalantari and Abbas Aflatoonian},
title = {In vivo differentiation of Mesenchymal Stem Cells (MSCs) in rat torsion testis},
booktitle = {4th Yazd International Congress and Student Award in Reproductive Medicine},
year = {2011},
location = {یزد, IRAN},
keywords = {Mesenchymal Stem Cells (MSCs); In vivo differentiation; Torsion testis},
}

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%0 Conference Proceedings
%T In vivo differentiation of Mesenchymal Stem Cells (MSCs) in rat torsion testis
%A Sabbaghi Mehrjardi, Mohammad Ali
%A Bahrami, Ahmad Reza
%A Behzad Feizzade
%A Seyed Mahdi Kalantar
%A Moghaddam Matin, Maryam
%A M Kalantari
%A Abbas Aflatoonian
%J 4th Yazd International Congress and Student Award in Reproductive Medicine
%D 2011

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