Title : ( A Comparison of Two Selectable Marker Gene Systems Used in the Transformation of Chickpea (Cicer arietinum L )
Authors: Nasrin Moshtaghi , Abdolreza Bagheri , Ahmad Sharifi ,
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Abstract
Different selectable marker genes are used in the genetic transformation of plants, but the efficiency of these genes in the selection of transgenic plants is different. In this study, we have used two different selectable marker gene systems in an established chickpea transformation protocol, and have compared the effectiveness of each selection system based on transformation efficiency. Using the herbicide resistance bar gene, together with a low concentration of the selective agent phosphinothricin, results in greater transformation efficiency than using the antibiotic resistance nptII gene with a high concentration of the selective agent kanamycin. In addition, modification of the rooting media and the use of a grafting method further improved the transformation efficiency of both selection systems. The transformation efficiency using the nptII and bar genes as selectable markers was 0.37% and 4.3%, respectively.
Keywords
, bar, marker gene, nptII, transformation, transgenic chickpea
@article{paperid:1022295,
author = {Moshtaghi, Nasrin and Bagheri, Abdolreza and Ahmad Sharifi},
title = {A Comparison of Two Selectable Marker Gene Systems Used in the Transformation of Chickpea (Cicer arietinum L},
journal = {Transgenic Plant Journal},
year = {2011},
volume = {5},
number = {1},
month = {June},
issn = {1749-0413},
pages = {67--71},
numpages = {4},
keywords = {bar; marker gene; nptII; transformation; transgenic chickpea},
}
%0 Journal Article
%T A Comparison of Two Selectable Marker Gene Systems Used in the Transformation of Chickpea (Cicer arietinum L
%A Moshtaghi, Nasrin
%A Bagheri, Abdolreza
%A Ahmad Sharifi
%J Transgenic Plant Journal
%@ 1749-0413
%D 2011
