It is known that, silver species show different effects in anti microbial and toxicity tests. Therefore it is necessary to use standard protocol on the basis of which both biology and nanotechnology parameters are considered. Some of these parameters are: diversity in nano-silver synthesis methods, type of stabilizer, size, shape, size distribution and concentration of nano particles, type of culture media and component of them, condition of tests, effect of light or uv-light to nano-silver and its antimicrobial potentials, role of time in tests, etc. Each of these factors could cause the result of one test to become specific to the parameters of that test i.e, experimental conditions, type of nano-silver and type of microorganism , and thus will not reliable extended to the experiments with different parameters

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