Introduction: One of the famous processes that in many research studied kinetically in vitro, is the elongation process. Elongation is meddle step in translation of proteins, during which peptide chain is made and elongated using some factors names ‘elongation factors’. In this work, detailed kinetics of the elongation cycle has been modeled. Material and methods: Kinetic simulations of the model were performed in ODEs form with Matlab simbiology Toolbox. Results: Using ordinary differential equations (ODEs) kinetics of the modelwas simulated. After simulation, various quasi-steady stateswere observed for the intermediate complexes that they have different amplitudes and times. During formation of the activated elongation factor Tu, some parameters have more effects than others; these parameters were assigned by sensitivity analysis and the mechanistic results were discussed. By incrementing Ts concentration production of Tu.GTP and also the rate of process are increased. Formation of pre translocation complex is sensitive to initial concentrations of the ribosome that were used during simulations. Finally effects of elongation factors concentration on the formation of post translocation complex were determined qualitatively by parameter scan. Conclusion: Here complete detailed model of elongation cycle has been established. The currentmodel stems fromin vitro measurements of elongation kinetics. The results are in agreement with experimental work

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