Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, ( ISI ), Volume (85), No (1), Year (2012-1) , Pages (79-84)

Title : ( Investigation of the behavior of HSA upon binding to amlodipine and propranolol: Spectroscopic and molecular modeling approaches )

Authors: Mohammad Reza Housaindokht , Zeinab Rouhbakhsh zaeri , Mahmood Bahrololoom , Jamshid Chamani , Mohamad Reza Bozorgmehr ,

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Abstract

The interaction between human serum albumin (HSA) and two drugs – amlodipine and propranolol – was investigated using fluorescence, UV absorption and circular dichroism (CD) spectroscopy. In addition, the binding site was established by applying molecular modeling technique. Fluorescence data suggest that amlodipine will quench the intrinsic fluorescence of HSA; whereas propranolol enhances the fluorescence of HSA. The binding constants for the interaction of amlodipine and propranolol with HSA were found to be 3.63 × 105 M−1 and 2.29 × 104 M−1, respectively. The percentage of secondary structure feature of each one of the HSA-bound drugs, i.e. the α-helix content, was estimated empirically by circular dichroism. The results indicated that amlodipine causes an increase, and that propranolol leads to a decrease in α-helix content of HSA. The spectroscopic analysis indicates that the binding mechanisms of the two drugs are different from each other. The data obtained by the molecular modeling study indicated that these drugs bind, with different affinity, to different sites located in subdomain IIA and IIIA.

Keywords

Human serum albumin; Amlodipine; Propranolol; Binding constants; Circular dichroism; Molecular modeling
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@article{paperid:1024370,
author = {Housaindokht, Mohammad Reza and Rouhbakhsh Zaeri, Zeinab and Bahrololoom, Mahmood and Jamshid Chamani and Mohamad Reza Bozorgmehr},
title = {Investigation of the behavior of HSA upon binding to amlodipine and propranolol: Spectroscopic and molecular modeling approaches},
journal = {Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy},
year = {2012},
volume = {85},
number = {1},
month = {January},
issn = {1386-1425},
pages = {79--84},
numpages = {5},
keywords = {Human serum albumin; Amlodipine; Propranolol; Binding constants; Circular dichroism; Molecular modeling},
}

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%0 Journal Article
%T Investigation of the behavior of HSA upon binding to amlodipine and propranolol: Spectroscopic and molecular modeling approaches
%A Housaindokht, Mohammad Reza
%A Rouhbakhsh Zaeri, Zeinab
%A Bahrololoom, Mahmood
%A Jamshid Chamani
%A Mohamad Reza Bozorgmehr
%J Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
%@ 1386-1425
%D 2012

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