Title : ( The interaction mode between DNA and salen-Co(III) N,N’-dipyridoxyl (1,4-butanediamine) Shiff-base complex )
Authors: Zahra Mashhadi Khoshkhoo , Mohammad Reza Housaindokht , Razieh Jalal , Hossein Eshtiagh Hosseini , sayyed hamid mir tabatabae , Masoud Mirzaei Shahrabi ,
Full TextAbstract
In this work, we studied the interaction of new salen-co(III) of N,N\\\'-dipyridoxyl (1,4-butanediamine) Schiff-base complex with DNA by melthing temperature, fluorescence spectrometry and gel electrophoresis techniques. This salen-Co(III) complex shows increase in melting temperature when bound to native calf thymus-DNA (CTDNA). The intersection point of the binding isotherm indicated a binding site size of 3 bp per bound complex molecule in Tris–HCl buffer. Upon adding the new salen-Co(III), the electrophoretic mobility of pTZ57R DNA plasmid becomes slower for both super coiled and open circular forms without any structural changes in DNA. The experimental results showed that the salen-Co(III) complex bound to DNA by intercalative mode.In this work, we studied the interaction of new salen-co(III) of N,N
