اولین کنگره ملی شیمی نوین ایران , 2011-05-04

Title : ( Immobilization of α-amylase onto magnetic nanoparticles by shaking method )

Authors: Razieh Jalal , zeinab mortazavi milani , Elaheh Kafshdare Goharshadi ,

Citation: BibTeX | EndNote

α-amylase was immobilized covalently on Fe3O4 magnetic nanoparticles via carbodiimide activation. The efficiency of immobilization and residual activity of the immobilized α-amylase was dependent upon the immobilization temperature and mass ratio of nanoparticles: carbodiimide: α-amylase. Confirmation of the binding was demonstrated by Fourier transform infrared (FTIR) spectroscopy. At room temperature, immobilized amylase displayed more binding efficiency and less residual activity compared with of 4°C, hence 4°C was chosen as optimum immobilization temperature. The 4:1:1 ratio displayed maximum residual activity therefore, it was chosen as a best ratio for immobilization. The optimum pH value for both free and immobilized amylase was at pH 5 but immobilized amylase exhibited more pH sensitivity at the pH values 6, 7 compared with free amylase and there were no differences in pH profiles for amylase that immobilized in room temperature or 4°C.

Keywords

, α-amylase, Magnetic nanoparticles, Immobilization,
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@inproceedings{paperid:1024718,
author = {Jalal, Razieh and Mortazavi Milani, Zeinab and Kafshdare Goharshadi, Elaheh},
title = {Immobilization of α-amylase onto magnetic nanoparticles by shaking method},
booktitle = {اولین کنگره ملی شیمی نوین ایران},
year = {2011},
location = {شیراز, IRAN},
keywords = {α-amylase; Magnetic nanoparticles; Immobilization; Carbodiimide},
}

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%0 Conference Proceedings
%T Immobilization of α-amylase onto magnetic nanoparticles by shaking method
%A Jalal, Razieh
%A Mortazavi Milani, Zeinab
%A Kafshdare Goharshadi, Elaheh
%J اولین کنگره ملی شیمی نوین ایران
%D 2011

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