Enhancement of dermal and epidermal regeneration is an extremely important goal for the treatment of wounds. The present study was evaluate the effects of autologous Platelet-rich plasma (PRP) on wound healing in dogs under treatment of dexamethasone. Ten male, mixed breed adult dogs, 2 to 4 years of age (2.9±0.69) and weighing between 27 to 35 kg (30.91±2.93). randomly arranged into two groups. In group one (five dogs), Six days before wounds creating, dogs received dexamethasone 0.5 mg/kg IM, every other day up to day 8 after wounding (8 treatments). Under general anesthesia, six 2*2 cm2 full-thickness skin wounds were created symmetrically on the back of each dog of both groups. Left side wounds, were left without treatment, and right side wounds, were treated topically with PRP gel. At day 7, 14 and 21 after wounding, biopsy was taken from the same corner of each wound for histopathologic evaluation. There were no significant differences between median of epithelialization, hemorrhage, necrotic tissue, fibrin clot, inflammatory infiltration, presence of dermal granulation tissue, fibroblast proliferation, arrangement of fibroblasts, collagen deposition scores, in the specimens from left and right wounds (P>0.05). The only significant finding was formation of collagen bundle that was greater in the PRP treated wounds in animals which received salin in contrast with treated wound in animal which recieved dexamethasone (P<0.05). In descriptive study epithelialization were greater in PRP treated wounds in contrast with non treated wounds in the animal which received salin (P=.063). The result of the present study demonstrated that topical application of platelet-rich plasma can not accelerate or improve the quality of repair of small granulating skin wounds on the back of normal dogs. This treatment may hold more promise for the management of chronic wounds that would benefit from a fresh source of mediators to accelerate the healing process. Likely mechanisms by which the gel led to facilitate wound healing are epithelialization and collagen formation.

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