African Journal of Microbiology Research, ( ISI ), Volume (5), No (30), Year (2011-12) , Pages (5489-5492)

Title : ( Detection of Clostridium tetani by fluorescent amplification based specific hybridization )

Authors: mohammad saadatmandzadeh , Hossein Eshghi , Mohammad Rahimizadeh , Mojtaba Sankian , Saeid Amel Jamehdar ,

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The etiologic agent of tetanus, Clostridium tetani, is an obligate anaerobic and spore-forming bacillus; and remains an important public health problem in developing countries. Although, the diagnosis is based mainly on history and presentation of tetanus symptoms. Some recent reports indicate that a patient may not manifest any symptoms of localized or generalized tetanus. However, one of the new approaches for accurate and rapid detection of microbial pathogens is a polymerase chain reaction (PCR) followed by fluorescence detection. In this report, we have described a fluorescent amplification based specific hybridization (FLASH) based PCR assay for detection of C. tetani. The results of the present study indicated that the FLASH based PCR assay appear to be a suitable and rapid method for identification of cultured isolates of C. tetani.

Keywords

, Clostridium tetani, molecular diagnosis, fluorescent amplification based specific hybridization (FLASH) polymerase chain reaction (PCR), fluorescent
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@article{paperid:1025035,
author = {Saadatmandzadeh, Mohammad and Eshghi, Hossein and Rahimizadeh, Mohammad and Mojtaba Sankian and Saeid Amel Jamehdar},
title = {Detection of Clostridium tetani by fluorescent amplification based specific hybridization},
journal = {African Journal of Microbiology Research},
year = {2011},
volume = {5},
number = {30},
month = {December},
issn = {1996-0808},
pages = {5489--5492},
numpages = {3},
keywords = {Clostridium tetani; molecular diagnosis; fluorescent amplification based specific hybridization (FLASH) polymerase chain reaction (PCR); fluorescent probe.},
}

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%0 Journal Article
%T Detection of Clostridium tetani by fluorescent amplification based specific hybridization
%A Saadatmandzadeh, Mohammad
%A Eshghi, Hossein
%A Rahimizadeh, Mohammad
%A Mojtaba Sankian
%A Saeid Amel Jamehdar
%J African Journal of Microbiology Research
%@ 1996-0808
%D 2011

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