Iranian Journal of Veterinary Medicine, Volume (5), No (4), Year (2011-12) , Pages (204-210)

Title : ( Isolation and characterization of Brachyspira species based on biochemical scheme and 16S rDNA partial sequencing )

Authors: Jamshid Razmyar , S. M. Peighambari , A. Barin ,

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Abstract

Abstract: Avian intestinal spirochetosis (AIS) is a disease of birds characterized by a marked colonization of the cecum and/or rectum with anaerobic intestinal bacteria of the genus Brachyspira. The purpose of the study was to determine the occurrence of Brachyspira spp from avian sources in Iran and to characterize selected isolates by biochemical and molecular methods. Out of 165 fecal samples obtained from commercial layer, broiler and broiler breeder chickens, turkeys and ostriches, 16 samples were positive in phase contrast microscopy examination and selective culture media. Biochemical scheme, polymerase chain reaction (PCR), and sequencing of partial sequence of 16S rDNA, revealed that all of isolates were Brachyspira pilosicoli, a broad spectrum host member of this genus that infects both humans and animals. This is the first report on isolation and identification of Brachyspira pilosicoli from birds in Iran.

Keywords

, Avian intestinal spirochetosis, Brachyspira pilosicoli, poultry, 16S rDNA, Iran.
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@article{paperid:1025221,
author = {Razmyar, Jamshid and S. M. Peighambari and A. Barin},
title = {Isolation and characterization of Brachyspira species based on biochemical scheme and 16S rDNA partial sequencing},
journal = {Iranian Journal of Veterinary Medicine},
year = {2011},
volume = {5},
number = {4},
month = {December},
issn = {2251-8894},
pages = {204--210},
numpages = {6},
keywords = {Avian intestinal spirochetosis; Brachyspira pilosicoli; poultry; 16S rDNA; Iran.},
}

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%0 Journal Article
%T Isolation and characterization of Brachyspira species based on biochemical scheme and 16S rDNA partial sequencing
%A Razmyar, Jamshid
%A S. M. Peighambari
%A A. Barin
%J Iranian Journal of Veterinary Medicine
%@ 2251-8894
%D 2011

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