European Journal of Plant Pathology, ( ISI ), Volume (129), No (3), Year (2011-2) , Pages (413-425)

Title : ( Characterization of Pectobacterium species from Iran using biochemical and molecular methods )

Authors: Sareh Baghaee Ravari , Heshmat Rahimaian , Masoud Shams-bakhsh , Emilia Lopez Solanilla , Maria Antunez-Lamas , Pablo Rodriguez-Palenzuela ,

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Characteristics of forty strains from macerated potato tubers and water-soaked lesions of some ornamental plants were studied in north parts of Iran. The causal organisms isolated from infected tissues were identified as Pectobacterium spp. based on their physiological and biochemical assays and confirmed by species and subspecies specific PCR and RFLP analysis of 16S- 23S intergenic transcribed spacer region. Artificial inoculation of isolates to their related hosts generated the same symptoms on potato and ornamental plants, from which the same bacteria were isolated and identified. We detected two groups of atypical isolates in this study. The first group from potato classified as Pectobacterium carotovorum subsp. carotovorum by phenotypic tests but was unable to elicit HR on tobacco leaves, to grow at 37 ˚c and to amplify the pel gene relevant to this subspecies. The second one from ornamental plants which was again characterized as Pectobacterium carotovorum subsp. carotovorum in biochemical assays, produced unique ITS-RFLP profile different from all of known Pectobacterium species and subspecies. Our findings based on phylogenetic analysis using concatenated partial sequences of housekeeping genes mdh and gapA, indicated the occurrence of P. wasabiae as a novel species in potato storages in Iran. Furthermore we detected a distinct clade of Pectobacterium spp. from some ornamental plants including Schlumbergera bridgesii, Syngonium podophyllum and Iris spp.

Keywords

, :housekeeping genes, ornamental plants, potato, Pectobacterium
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@article{paperid:1026738,
author = {Baghaee Ravari, Sareh and Heshmat Rahimaian and Masoud Shams-bakhsh and Emilia Lopez Solanilla and Maria Antunez-Lamas and Pablo Rodriguez-Palenzuela},
title = {Characterization of Pectobacterium species from Iran using biochemical and molecular methods},
journal = {European Journal of Plant Pathology},
year = {2011},
volume = {129},
number = {3},
month = {February},
issn = {0929-1873},
pages = {413--425},
numpages = {12},
keywords = {:housekeeping genes; ornamental plants; potato; Pectobacterium strains},
}

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%0 Journal Article
%T Characterization of Pectobacterium species from Iran using biochemical and molecular methods
%A Baghaee Ravari, Sareh
%A Heshmat Rahimaian
%A Masoud Shams-bakhsh
%A Emilia Lopez Solanilla
%A Maria Antunez-Lamas
%A Pablo Rodriguez-Palenzuela
%J European Journal of Plant Pathology
%@ 0929-1873
%D 2011

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