Kragujevac Journal of Science, ( ISI ), Volume (34), No (34), Year (2012-4) , Pages (71-78)

Title : ( Phytochemical screening, antimicrobial and antioxidant activities of Anabasis aphylla extracts )

Authors: Abollfazl Shakeri , Nourallah Hazeri , Jafar Vlizadeh , Ali Ghasemi , Fatemeh Zaker Tavallaie ,

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Abstract

ABSTRACT. The present study was designed to evaluate the phytochemical screening, antimicrobial and antioxidant activities of Anabasis aphylla L. extracts. Phytochemical screening revealed the presence of alkaloid, flavonoid, saponin, terpenoid, steroid and sterols in the extracts of aerial parts of A. aphylla. The dry aerial part of A. aphylla was extracted with methanol, then further fractioned with n-hexan, ethyl acetate, n-butanol, and water. Four fractions (methanol, ethyl acetate, n- butanol, and water) were examined for antimicrobial activity by using disc diffusion assay. The microbial strains were exposed to the following four different concentrations of extracts: 1, 10, 25 and 100 mg/ml. There has been an increasing effect on microbial growth inhibition with increasing concentration of the extracts. The largest zone of inhibition was obtained with n-butanol extract against Aspergillus niger (20mm). The antimicrobial activity of extracts was compared with the standard antibiotics like amoxicillin and ketoconazol (100 µg/disc). Furtheremore, the antioxidant activity of two fractions (methanol and ethyl acetate) were tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene/linoleic acid assays. The extracts showed no antioxidant activity in DPPH method. In βcarotene/linoleic acid test, the ethyl acetate (100 mg/ml) showed higher antioxidant activity (81.8%).

Keywords

, phytochemical, antimicrobial activity, antioxidant activity, Anabasis aphylla.
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@article{paperid:1028752,
author = {Abollfazl Shakeri and Nourallah Hazeri and Jafar Vlizadeh and Ali Ghasemi and Zaker Tavallaie, Fatemeh},
title = {Phytochemical screening, antimicrobial and antioxidant activities of Anabasis aphylla extracts},
journal = {Kragujevac Journal of Science},
year = {2012},
volume = {34},
number = {34},
month = {April},
issn = {1450-9636},
pages = {71--78},
numpages = {7},
keywords = {phytochemical; antimicrobial activity; antioxidant activity; Anabasis aphylla.},
}

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%0 Journal Article
%T Phytochemical screening, antimicrobial and antioxidant activities of Anabasis aphylla extracts
%A Abollfazl Shakeri
%A Nourallah Hazeri
%A Jafar Vlizadeh
%A Ali Ghasemi
%A Zaker Tavallaie, Fatemeh
%J Kragujevac Journal of Science
%@ 1450-9636
%D 2012

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