Title : ( Comparative morphological and molecular study of Iranian populations of Heterodera filipjevi (Madzhidov, 1981) Stelter,1984 and other members of ‘H. avenae group’ )
Authors: abas mokaram hesar , Esmat Mahdikhani Moghadam , Zahra Tanha Maafi , Javad Karimi ,
Full TextAbstract
Populations of ‘H. avenae group’ were collected from sugar beet fields in Khorasan Razavi province (Iran) and identified on the basis of both morphological and morphometrical characters. PCR-RFLP and sequencing of rDNA-ITS regions were used to confirm the previous identification. Principal Components Analysis (PCA) of four morphometric characters of second-stage juveniles and vulval cones showed the overlapping between different species and populations, while UPGMA cluster analysis was used for grouping. Several populations of H. filipjevi, H. mani and H. avenae were grouped close together and PCA analysis demonstrated high overlapping between some populations of these species. The morphology of stylet knobs in H. filipjevi presented high intraspecific variability, hence this character is not useful to separate it from other closely related forms.
Keywords
, Heterodera avenae group’, identification, ITS-rDNA, morphometrics, PCA, RFLP
@article{paperid:1029140,
author = {Mokaram Hesar, Abas and Mahdikhani Moghadam, Esmat and Zahra Tanha Maafi and Karimi, Javad},
title = {Comparative morphological and molecular study of Iranian populations of Heterodera filipjevi (Madzhidov, 1981) Stelter,1984 and other members of ‘H. avenae group’},
journal = {Journal of Nematode Morphology and Systematics},
year = {2012},
volume = {15},
number = {1},
month = {July},
issn = {1139-5192},
pages = {1--11},
numpages = {10},
keywords = {Heterodera avenae group’; identification; ITS-rDNA; morphometrics; PCA; RFLP},
}
%0 Journal Article
%T Comparative morphological and molecular study of Iranian populations of Heterodera filipjevi (Madzhidov, 1981) Stelter,1984 and other members of ‘H. avenae group’
%A Mokaram Hesar, Abas
%A Mahdikhani Moghadam, Esmat
%A Zahra Tanha Maafi
%A Karimi, Javad
%J Journal of Nematode Morphology and Systematics
%@ 1139-5192
%D 2012
