Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, ( ISI ), Volume (98), No (8), Year (2012-8) , Pages (1089-1100)

Title : ( Interaction between holo transferrin and HSA–PPIX complex in the presence of lomefloxacin: An evaluation of PPIX aggregation in protein–protein interactions )

Authors: Zohreh Sattar , Hediye Iranfar , Ahmad Asoodeh , Mohammad Reza Saberi , Mahboobeh Mazhari , Jamshidkhan Chamani ,

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Human serum albumin (HSA) and holo transferrin (TF) are two serum carrier proteins that are able to interact with each other, thereby altering their binding behavior toward their ligands. During the course of this study, the interaction between HSA–PPIX and TF, in the presence and absence of lomefloxacin (LMF), was for the first time investigated using different spectroscopic and molecular modeling techniques. Fluorescence spectroscopy experiments were performed in order to study conformational changes of proteins. The RLS technique was utilized to investigate the effect of LMF on J-aggregation of PPIX, which is the first report of its kind. Our findings present clear-cut evidence for the alteration of interactions between HSA and TF in the presence of PPIX and changes in drug-binding to HSA and HSA–PPIX complex upon interaction with TF. Moreover, molecular modeling studies suggested that the binding site for LMF became switched in the presence of PPIX, and that LMF bound to the site IIA of HSA. The obtained results should give new insight into research in this field and may cast some light on the dynamics of drugs in biological systems.

Keywords

, HSA Holo transferrin Fluorescence quenching Zeta, potential Protein–protein
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@article{paperid:1029263,
author = {Zohreh Sattar and Hediye Iranfar and Asoodeh, Ahmad and Mohammad Reza Saberi and Mahboobeh Mazhari and Jamshidkhan Chamani},
title = {Interaction between holo transferrin and HSA–PPIX complex in the presence of lomefloxacin: An evaluation of PPIX aggregation in protein–protein interactions},
journal = {Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy},
year = {2012},
volume = {98},
number = {8},
month = {August},
issn = {1386-1425},
pages = {1089--1100},
numpages = {11},
keywords = {HSA Holo transferrin Fluorescence quenching Zeta-potential Protein–protein interaction},
}

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%0 Journal Article
%T Interaction between holo transferrin and HSA–PPIX complex in the presence of lomefloxacin: An evaluation of PPIX aggregation in protein–protein interactions
%A Zohreh Sattar
%A Hediye Iranfar
%A Asoodeh, Ahmad
%A Mohammad Reza Saberi
%A Mahboobeh Mazhari
%A Jamshidkhan Chamani
%J Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
%@ 1386-1425
%D 2012

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