بیستمین کنگره گیاهپزشکی ایران , 2012-08-25

عنوان : ( شیوع Wolbachia در برخی گونه های مگس های میوه خراسان:مطالعه ای با رویکرد MLST )

نویسندگان: جواد کریمی , ریحانه درسوئی ,
فایل: Full Text

استناددهی: BibTeX | EndNote

چکیده

Wolbachia is a widespread endosymbiont of insects with diverse range of biological effects on its hosts. We studied the prevalence of Wolbachia in some important species of tephritids in the Iran. Among different populations of five fruit fly species, Dacus ciliatus (cucurbit fly), Rhagoletis cerasi (cherry fruit fly), Ceratitis capitata (Mediterranean fruit fly), Myiopardalis pardalina (melon fly) and Carypomya vesuviana (jujube fly), two species including R. cerasi and C. vesuviana showed different levels of Wolbachia infection with two new strains, namely wRc1 and wCv1, respectively. C. vesuviana is introduced here as a new host for Wolbachia. Genotyping of Wolbachia strains in 12 populations of five fruit fly species, using multilocus sequence typing (MLST) and wsp gene sequence showed occurrence of two new strains as well new strain type assigned as ST 277 belong to the A supergroup as symbiont with C. vesuviana. Prevalence status of Wolbachia was different among infected species. While we showed fixed infection of cherry fruit fly with Wolbachia, this condition was variable for Carypomya vesuviana . Here, 12 barcodes under 5 species of Iranian tephritids are added to the database of DNA barcodes. Inter-intra specific differences among COI sequences showed a clear gap barcoding between most fruit flies. Considering the research trend about Wolbachia, this knowledge will promise for a new approach toward application of Wolbachia through cytoplasmic incompatibility (CI) for management of pest species of tephritids. Current work is the first attempt using MLST approach toward characterization of insect endosymbiont in Iran.

کلمات کلیدی

, مگس های میوه, Wolbachia, MLST
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@inproceedings{paperid:1029464,
author = {کریمی, جواد and درسوئی, ریحانه},
title = {شیوع Wolbachia در برخی گونه های مگس های میوه خراسان:مطالعه ای با رویکرد MLST},
booktitle = {بیستمین کنگره گیاهپزشکی ایران},
year = {2012},
location = {ايران},
keywords = {مگس های میوه، Wolbachia، MLST},
}

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%0 Conference Proceedings
%T شیوع Wolbachia در برخی گونه های مگس های میوه خراسان:مطالعه ای با رویکرد MLST
%A کریمی, جواد
%A درسوئی, ریحانه
%J بیستمین کنگره گیاهپزشکی ایران
%D 2012

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