There are many sports drinks, gels and powders that claim to assist increasing muscle mass. However, negative side effects of these compounds like kidney damage, loss of calcium, gout, dehydration and etc, have threatened the health of users. Although producer claim that all mass gain products are prepared naturally but as they are presented in new chemical form, their toxicity is uncertain. There are several tools to evaluate the probability of toxicity or mutagenicity of a chemical compound but microorganisms are easy to work with and also inexpensive. Bacterial biosensors are mainly used for either the control of general toxicity or specifically if the target to be measured for a biodegradation pathway, metal resistance or stress. A reporter reaction like bioluminescence is often coupled to gene expression to allow a fast, sensitive and non-destructive signal. Therefore luxAB gene encoding luciferase enzyme contained on pUT miniTn5 plasmid transformed into E.coli SM10 λpir and their stability and light intensity were characterized and confirmed. Then 0.1, 0.2, 0.4, and 0.6 g/ml water solution of mass gain diet samples comprising carbo complex, amino, supercreatine and protein were prepared and according to standard protocol light intensity were measured on a luminometer from a cuvete containing mixture of 450 μl of water solution of chemical diet and 50 μl of biosensor E.coli SM10 S1. Results showed that by increasing of amino and protein diet concentration luminescence light intensity decreased in regular order. However, various concentration of supercreatine did not make significant changes in the amount of light output of E.coli SM10 S1. Only 0.4 and 0.6 g/ml of carbo complex decreased relative luminescence values compared with positive control. These results show that further investigation on compounds especially those with higher effect on light intensity such as amino and protein should be accomplished to verify the usage of such compounds. It is also concluded that application of luminescent microbial biosensor for investigation on the quality of such products is new idea.

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