Black zira (Bunium persicum) is a medical and spice plant that is native in a limited area of the West of Asia and the Eastern parts of Iran. In this experiment the growth of Black zira from germination to the seeding and tuberization were investigated in vitro. Germination of black zira seeds on a simple medium containing 0.5 percent sugar was better than others. It happened in dark at 4 °C after at least 3 - 4 weeks. The seedlings were cultured on MS basal medium and then stored at 25°C and 16 hrs photoperiod. At this conditions cotyledons appeared and then a single tuber formed at the end of the root. The true leaves appeared one month after tuber formation. The effect of different concentrations of sugar on tubers size was found to be non significant. While the MS medium with different levels of nitrogen and ammonium to nitrate ratios were significant. The MS medium containing 30 mM nitrogen was more effective than 60 mM on the tuber size (length and diameter). The production of larger tubers requires both sources of nitrogen (ammonium and nitrate), but nitrate source was found to be more effective. The effect of different strengths of MS medium (MS, ½ MS, MS with ½ NO3 content) on weight, length and diameter of tuber was statistically non significant, but in all of traits, ½ MS medium had higher means.

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