Bunium persicum is one of the important medicinal and aromatic plants in Iran that belongs to the Apiaceae family. Seeds of this plant contain valuable components including essential oil. In this research the cells from cell suspension cultures and seed of Black zira were extracted by supercritical fluid (SCF), hydrodistillation and solvent methods and analyses by GC. The germinated seeds were the explants for induction of callus on MS medium supplemented with 2 mg/l 2,4-D and 0.5 mg/l kin (A), 2 mg/l NAA and 0.5 mg/l BA (B) and followed by cell suspension cultures establishment. Results of cell suspension cultures showed that the cells on B medium have growth rate higher than A. It was also found that no essential oil was obtained from hydrodistillation of cells, while SCF and solvent methods were successful in essential oil extraction from cells. According to GC analysis, cuminaldehyde as one of the Black zira essential constitute is not detected in the SCF cell and seed samples, while it was present in solvent extracts of both cell and seed samples. Cuminaldehyde concentration in cell and seed solvent extractions was 4.65% and 18.61% respectively [1- 6].

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