Title : ( Establishment of a Short-term Storage Method via Encapsulation of PLBs in Phalaenopsis bellina (Rchb.f.) Christenson. Seed Science and Technology )
Authors: Amir Ali Khoddamzadeh , U. R. Sinniah , M. A. Kadir , S. B. Kadzimin , M. Mahmood , S. Sreeramanan ,
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Abstract
Protocorm-like bodies (PLBs) of Phalaenopsis bellina (Rchb. f.) Christenson initiated from leaf explants, followed by proliferation on modifi ed semi-solid, half-strength, MS media, were used in this study. PLBs measuring 3 - 4 mm in diameter were selected for encapsulation with different concentrations of sodium alginate (3, 4 and 5%) and calcium chloride (25, 50, 75 and 100 mM), with non-encapsulated PLBs as the control. The highest survival after 2 weeks incubation at 5°C was 70% from the PLBs encapsulated with 4% sodium alginate plus 75 mM calcium chloride. Storage at 5, 15 and 25°C for up to 60 days, led to a decline in the viability of PLBs with a greater decline when stored at higher temperature. The highest PLB fresh weight of 0.36 g also occurred after storage at 5°C and the lowest fresh weight (0.19 g) occurred after 60 d at 25°C when viability was 0%.
Keywords
, short-term storage method, encapsulation, synthetic seeds, somatic embryo, PLB
@article{paperid:1030289,
author = {Khoddamzadeh, Amir Ali and U. R. Sinniah and M. A. Kadir and S. B. Kadzimin and M. Mahmood and S. Sreeramanan},
title = {Establishment of a Short-term Storage Method via Encapsulation of PLBs in Phalaenopsis bellina (Rchb.f.) Christenson. Seed Science and Technology},
journal = {Seed Science and Technology},
year = {2011},
volume = {39},
number = {3},
month = {October},
issn = {0251-0952},
pages = {697--702},
numpages = {5},
keywords = {short-term storage method; encapsulation; synthetic seeds; somatic embryo; PLB},
}
%0 Journal Article
%T Establishment of a Short-term Storage Method via Encapsulation of PLBs in Phalaenopsis bellina (Rchb.f.) Christenson. Seed Science and Technology
%A Khoddamzadeh, Amir Ali
%A U. R. Sinniah
%A M. A. Kadir
%A S. B. Kadzimin
%A M. Mahmood
%A S. Sreeramanan
%J Seed Science and Technology
%@ 0251-0952
%D 2011
