International Journal of Peptide Research and Therapeutics, ( ISI ), Volume (18), No (4), Year (2012-10) , Pages (353-360)

Title : ( Structure and ACE-Inhibitory Activity of Peptides Derived from Hen Egg White Lysozyme )

Authors: Mina Memarpoor-Yazdi , Ahmad Asoodeh , JamshidKhan Chamani ,

Citation: BibTeX | EndNote

Angiotensin I-converting enzyme plays an important role in hypertension and therefore its inhibition is considered to be a useful procedure in the prevention of hypertension. Two novel ACE inhibitory peptides were purified and identified from the papain-trypsin hydrolysate of hen egg white lysozyme using reverse phase-high performance liquid chromatography. The sequences of identified peptides were NTDGSTDYGILQINSR (MW: 1,753.98 ± 0.5 Da) and VFGR (MW: 459.26 ± 0.5 Da), which were named F2 and F9 peptide, respectively. Analyses of the far-UV CD spectra of ACE in the absence and presence of the F2 peptide revealed ACE secondary structural changes. In the presence of the F2 peptide, a loss of helical content of ACE was observed, which can lead to decrease of the enzymatic activity. Lineweaver–Burk plots show that the identified peptides both act as non-competitive ACE inhibitors. These findings would be helpful on the understanding of interaction between ACE and its inhibitory peptides.

Keywords

, RP-HPLC, ACE inhibitory activity, Inhibition pattern, Circular dichroism
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@article{paperid:1030472,
author = {Mina Memarpoor-Yazdi and Asoodeh, Ahmad and JamshidKhan Chamani},
title = {Structure and ACE-Inhibitory Activity of Peptides Derived from Hen Egg White Lysozyme},
journal = {International Journal of Peptide Research and Therapeutics},
year = {2012},
volume = {18},
number = {4},
month = {October},
issn = {1573-3149},
pages = {353--360},
numpages = {7},
keywords = {RP-HPLC; ACE inhibitory activity; Inhibition pattern; Circular dichroism spectroscopy},
}

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%0 Journal Article
%T Structure and ACE-Inhibitory Activity of Peptides Derived from Hen Egg White Lysozyme
%A Mina Memarpoor-Yazdi
%A Asoodeh, Ahmad
%A JamshidKhan Chamani
%J International Journal of Peptide Research and Therapeutics
%@ 1573-3149
%D 2012

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