Background: The resistance of bacteria to current medicine and emergence of new bacterial diseases urges us to synthesis new drugs. The anti-bacterial features of pyridinium compounds are proven and in this study a new derivative of compound, called 1, 1 -(Ethane-1,2-diyl)dipyridinium dichromate(VI), is synthesized and its anti-bacterial feature has been tested on some standard bacteria using microplate method. Materials and Methods: Above mentioned compound was dissolved in Muller-Hinton Broth medium and was diluted to a certain amount. Certain amounts of that compound and the bacteria were added to microplate wells and after 17 hours of incubation in a temperature of 37 degrees,the Minimum Inhibitory Concentration (MIC) was measured using ELISA Reader device and the Minimum Bactericidal Concentration (MBC) was determined using microcolony assay on agar medium. Results: The results revealed that the growth of gram negative bacteria Pseudomonas aeruginosa and Escherichia coli is inhibited in 750 µg/ml density; whereas, for Staphylococcus aureus 1500 µg/ml density was inhibitory. Also MBC was matched with MIC. Conclusion: The new synthesized compound has antimicrobial effect on both gram positive and gram negative bacteria, however, the gram negative bacteria were more sensitive than gram positive.

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