Nepeta binaludensis Jamzad is a rare and highly endangered ethnomedicinal plant of lamiaceae family which grows in Khorasan province in Northeast of Iran. Afield experiment was conducted with biennial cultivation of the plant during 2006 and 2007 at research center of college of agriculture in Ferdowsi University of Mashhad, to assay its domestication performance. To evaluate the effects of agronomical conditions on its establishment, phenology, yield, morphological and phytochemical properties, different experiments including irrigation intervals (7, 14, 21 and 28 days), amounts of cow manure (10,20,30 and 40 ton/ha) and plant spacing (two row spacing of 50 and 75 cm and two inter-row spacing of 25 and 50 cm) were studied. Results indicated that this species can well establish and grow in agricultural system. The highest biomass, height, plant diameter, essential oil yield and Eucalyptol (1,8 Cineole) content were obtained at 7 days irrigation interval. The effects of cow manure on biomass, essential oil content and chemical components were not statistically significant. The whole growth period of this species was nearly 206-214 days which was equivalent to 2792.2-2826.2 GDD. Plant distances did not affect biomass and essential oil content significantly.

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