Background: Recent studies suggest that an elevated serum ferritin concentration is considered an dependent factor associated with increased risk cardiovascular disease. The aim of this research is finding of the effect of six months aerobic exercise on serum ferritin levels in untrained middle-aged women. Methods: Nineteen healthy female middle-aged were selected by convenience sampling method and were randomly divided into two experimental (n=11) and control (n=8) groups. The exercise protocol included aerobic exercise training lasted for 6 months and 3 sessions per week and every session lasted for 60 minutes and with intensity of 55-65 percent of maximum heart rate reserve (MHR). Blood samples were taken to measure serum ferritin before and after aerobic training period. General linear- Repeated measures (GL-RM) was used to comparing of within, Interactive and between means groups. The level of significance was set at P< 0.05. Results: Weight, BMI, body far percent, WHR in exercise group towards the end of period of the training, but this changes was not signifi cantly. Results showed a variance between group WHR is signifi cant (P<0.05). In addi tion, during the training, there was no significant change in serum Ferritin levels in both groups. Conclusion: Six months of aerobic exercise does not induce significant change in serum levels of Ferritin, while these levels reduced in middle-aged women. We believe that prolong exercise, due to reduce in serum ferritin. Although more research, as well as basic research and large-scale epidemiological studies, is required to totally assess the association between Ferritin concentrations and prolong exercise.

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