Food and Chemical Toxicology, ( ISI ), Volume (49), No (1), Year (2011-1) , Pages (1102-1109)

Title : ( Curcumin potentiates doxorubicin-induced apoptosis in H9c2 cardiac musclecells through generation of reactive oxygen species )

Authors: Leila Hosseinzadeh , Javad Behravan , Fatemeh Mosaffa , Gholamreza Bahrami , Ahmad Reza Bahrami , Gholamreza Karimi ,

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Doxorubicin (DOX) is a widely used chemotherapy agent. The major adverse effect of DOX treatment in cancer patients is the onset of cardiomyopathy and heart failure. Reactive oxygen species (ROS) are proposed to be responsible for DOX cardiotoxicity. Curcumin, a natural compound extracted from Curcuma Longa L., is known for its anti-oxidant properties. It has been identified as increased apoptosis in several cancer cell lines in combination with doxorubicin, but there are few studies about the effect of curcumin and doxorubicin on normal cardiac cells. Therefore, we evaluated the effects of curcumin on apoptosis induced by DOX in cardiac muscle cells. Preteatment with curcumin significantly increased DOX-induced apoptosis of cardiac muscle cells through down regulation of Bcl-2, up-regulation of caspase-8 and caspase-9. The Bax/Bcl-2 ratio increased significantly after 1 h pretreatment with curcumin. As well, curcumin increases ROS generation by DOX. In response to DOX, NF-jB was activated. However, curcumin was able to inhibit NF-jB activation. In conclusion, our results indicated that pretreatment with nontoxic concentrations of curcumin sensitized H9c2 cells to DOX-mediated apoptosis by generation of ROS.

Keywords

Doxorubicin Curcumin H9c2 Oxidative stress Apoptosis Nuclear
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@article{paperid:1031170,
author = {Leila Hosseinzadeh and Javad Behravan and Fatemeh Mosaffa and Gholamreza Bahrami and Bahrami, Ahmad Reza and Gholamreza Karimi},
title = {Curcumin potentiates doxorubicin-induced apoptosis in H9c2 cardiac musclecells through generation of reactive oxygen species},
journal = {Food and Chemical Toxicology},
year = {2011},
volume = {49},
number = {1},
month = {January},
issn = {0278-6915},
pages = {1102--1109},
numpages = {7},
keywords = {Doxorubicin Curcumin H9c2 Oxidative stress Apoptosis Nuclear factor–jB},
}

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%0 Journal Article
%T Curcumin potentiates doxorubicin-induced apoptosis in H9c2 cardiac musclecells through generation of reactive oxygen species
%A Leila Hosseinzadeh
%A Javad Behravan
%A Fatemeh Mosaffa
%A Gholamreza Bahrami
%A Bahrami, Ahmad Reza
%A Gholamreza Karimi
%J Food and Chemical Toxicology
%@ 0278-6915
%D 2011

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