Background: Several studies show that doing high intensity activity lead to changes in anabolic and catabolic salivary hormone levels. The aim of this study was determined the levels of salivary cortisol and testosterone during futsal game on futsal players. Materials and Methods: Twenty two male futsal players from Iranian national league teams with age 24.09±2.28 yr, height 174.0±5.92 cm, weight 68.72±5.62 kg, BMI 22.71±1.66 kg/m2 volunteered to take part in this study. Samples were collected before and after the first half, before and after the second half. General linear repeated measures (GL-RM) and Post-hoc Bonferroni test was used to comparing variations of within and between means phases. The level of significance was set at P<0.05. Results: One session futsal game had a significant effect on salivary cortisol concentration (P<0.001). Hence, these changed have increased rather than to the baseline levels; the peak of this change was observed before the second half. Salivary testosterone levels during this period increased significantly (P<0.001). Conclusion: Futsal games leads to the salivary cortisol and testosterone levels increased significantly in futsal’ male. In addition, a futsal game maybe leads to imbalance of anabolic to catabolic ratio in athletes.

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