An experiment was conducted to investigate the effects of fruit hydroalcoholic extract of Withania coagulans (WC) and 1, 25-dihydroxycholecalciferol on bone mechanical and histological properties of male chickens. Diets were arranged factorially (2×3×2) consisted of a positive control with adequate Ca (10.4 g/kg diet) and                            g/kg diet), three levels of Withania coagulans (0, 100 and 200 mg/kg diet), and two levels of 1, 25- dihydroxycholecalciferol    !   No main effects of experimental treatments on tibia physical characteristics and bone mineralization were observed except for tibia diameter. Reducing the dietary Ca level by 30 % decreased tibia diameter (P<0.05) at 42 day of age. Supplementation of 1, 25-dihydroxycholecalciferol increased energy requirement for bone fracture, bone proximal mineralize width and serum Ca concentration (P<0.05). Supplementation of 100 mg/kg WC significantly increased shear force (P<0.05) and fracture energy (P<0.01) at 42 day of age. The effects of dietary supplemental WC on fracture deflection only approached significant (P=0.053). At 21 day of age birds provided with 100 mg/kg of WC had a significant higher cortical thickness (P<0.05) and tended to increase at 42 day. The results of this experiment indicated that supplementation of 100 mg/kg fruit hydroalcoholic extract of Withania coagulans exhibit beneficial effects on bone characteristics (mechanical property and cortical thickness) without adverse effects on productive performance.

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