Isabgol (Plantago ovata L.) is an annual medicinal plant that the dried seeds and the seed husks are demulcent, emollient and laxative. In order to evaluate the effect of different cow manure levels on yield and mucilage content of isabgol, a field experiment was performed based on randomized completed block design with three replications, at Agricultural Research Station, College of Agriculture, Ferdowsi University of Mashhad, during growing season of 2011-2012. Treatments included four levels of cow manure (zero (C0), 5 (C5), 10 (C10) and 15 (C15) t.ha-1). Spike length, biological yield, seed yield, and mucilage content of isabgol were measured accordingly. The results showed that the different amounts of cow manure had significant impact (p≤0.05) on spike length, biological yield, seed yield and mucilage content of isabgol. The maximum seed yield was observed in C15 with 539.3 kg.ha-1 and the minimum was for C0 with 475.8 kg.ha-1. By increasing in cow manure level from zero to 15 t.ha-1 enhanced mucilage content up to 19%.

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