Title : ( Molecular and morphological characterization of Pristionchus pacificus (Nematoda: Rhabditida: Neodiplogastridae), a new record of an entomophilic nematode from Iran )
Authors: Mahnaz Hasani Kakhki , Javad Karimi , Ebrahim Shokoohi ,
Full TextAbstract
During a survey of entomopathogenic nematodes from the Mashhad region, in northeastern Iran, an entomophilic nematode was isolated using the Galleria trap method. Morphological characterization showed that this isolate belongs to the family Neodiplogastridae. Detailed morphological and morphometric characterization including SEM data fit with Pristionchus pacificus Sommer, Carta, Kim et Sternberg, 1996, representing the first record of this genus and species from Iran. Iranian specimens of P. pacificus FUM 5 are characterized by the body length (0.69–0.83 mm), the stoma (6–10 μm) bearing a dorsal tooth and a conical elongated tail (146–220 μm). Molecular analyses using 18S and ITS rDNA genes further support this identification. Measurements, illustrations, and SEM photographs of the Iranian isolate are provided and the phylogenetic position of the species is discussed.
Keywords
Pristionchus pacificus; SEM; ITS; 18S rDNA; Iran
@article{paperid:1035375,
author = {Hasani Kakhki, Mahnaz and Karimi, Javad and Ebrahim Shokoohi},
title = {Molecular and morphological characterization of Pristionchus pacificus (Nematoda: Rhabditida: Neodiplogastridae), a new record of an entomophilic nematode from Iran},
journal = {Biologia},
year = {2013},
volume = {68},
number = {5},
month = {July},
issn = {0006-3088},
pages = {910--917},
numpages = {7},
keywords = {Pristionchus pacificus; SEM; ITS; 18S rDNA; Iran},
}
%0 Journal Article
%T Molecular and morphological characterization of Pristionchus pacificus (Nematoda: Rhabditida: Neodiplogastridae), a new record of an entomophilic nematode from Iran
%A Hasani Kakhki, Mahnaz
%A Karimi, Javad
%A Ebrahim Shokoohi
%J Biologia
%@ 0006-3088
%D 2013
