Cytotherapy, ( ISI ), Volume (15), No (8), Year (2013-8) , Pages (951-960)

Title : ( Evidence for crossing the blood barrier of adult rat brain by human adipose-derived mesenchymal stromal cells during a 6-month period of post-transplantation )

Authors: Aliakbar Haddad-Mashadrizeh , Ahmad Reza Bahrami , Maryam Moghaddam Matin , Mohammad Amin Edalatmanesh , Alireza Zomorodipour , Ali Fallah , Mossa Gardaneh , Naghmeh Ahmadian Kia , Naser Sanjarmoosavi ,

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Background aims. Therapeutic promises of adult stem cells have been overshadowed by an elicited immune response, low maintenance of implanted cells or concerns regarding their migration to non-target sites. These problems might be lessened by the use of immune privilege cells and tissues for implantation. Methods. In this study, human adipose-derived mesenchymal stromal cells (hADMSCs) were stably transfected with a vector containing Turbo green fl uorescent protein (GFP) and JRed, which allows tracing the cells after transplantation. Labeled hADMSCs were transplanted into the adult rat brain followed by assessment of their survival and migration during 6 months after transplantation. Results.Results indicate that there were no postsurgical complications, and the animals thrived after transplantation. The lesions of the surgical process were remarkable at the fi rst weeks, and a high number of transplanted cells were accumulated around them. Cell populations declined over time as they partly migrated away from the injection sites; nonetheless, they were detectable at each examination time point. Although the cells could survive and remain at the injection site for up to 6 months, some of them drifted to spleen, which is an indication of their ability to cross the blood-brain barrier. Conclusions. Despite the high survival rate of hADMSCs in the xenogenic condition, which is an ideal criterion in cell therapy, irregular migration tendency must be handled with caution.

Keywords

, cell migration, cell survival, immune privilege, mesenchymal stromal cells, stem cell therapy,
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@article{paperid:1035453,
author = {Haddad-Mashadrizeh, Aliakbar and Bahrami, Ahmad Reza and Moghaddam Matin, Maryam and Mohammad Amin Edalatmanesh and Alireza Zomorodipour and Ali Fallah and Mossa Gardaneh and Naghmeh Ahmadian Kia and Naser Sanjarmoosavi},
title = {Evidence for crossing the blood barrier of adult rat brain by human adipose-derived mesenchymal stromal cells during a 6-month period of post-transplantation},
journal = {Cytotherapy},
year = {2013},
volume = {15},
number = {8},
month = {August},
issn = {1465-3249},
pages = {951--960},
numpages = {9},
keywords = {cell migration; cell survival; immune privilege; mesenchymal stromal cells; stem cell therapy; xenotransplantation},
}

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%0 Journal Article
%T Evidence for crossing the blood barrier of adult rat brain by human adipose-derived mesenchymal stromal cells during a 6-month period of post-transplantation
%A Haddad-Mashadrizeh, Aliakbar
%A Bahrami, Ahmad Reza
%A Moghaddam Matin, Maryam
%A Mohammad Amin Edalatmanesh
%A Alireza Zomorodipour
%A Ali Fallah
%A Mossa Gardaneh
%A Naghmeh Ahmadian Kia
%A Naser Sanjarmoosavi
%J Cytotherapy
%@ 1465-3249
%D 2013

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